Post-doctoral fellow in Epigenetics and Immunity

St. Anne’s University Hospital Brno International Clinical Research Center

We are now looking for a motivated Post-doctoral computational biologist with background in epigenetics and/or immunology, to investigate key epigenetic mechanisms involved in the protection and in the regeneration of hematopoietic stem cells upon damage. The correct cultural background (training in Computational Biology / Computer Science / Statistics or a mix of the three) and a strong interest in working with high throughput data will be considered at the same level of specific experience in the above-mentioned fields.

Interested? Join our team at the International Clinical Research Center of St. Anne’s University Hospital Brno (FNUSA-ICRC)!

Desired starting date: 01.03.2017 (negotiable)

Type of contract: full-time assignment (1 FTE)

Job Description - you will:

  • analyze large scale transcriptomic and epigenomic data from various sources (animal, human, cells and tissues) generated in the lab or retrieved from public databases.
  • use various software packages, open sources and commercial one.
  • be able to graphically represent omic data in visually appealing fashions.
  • interact with scientists specialized in diverse topics of bio-medicine, including cancer and immunity.

Your future workplace:

You will join the group led by Dr. Manlio Vinciguerra, PhD, within the Center of Translational Medicine (CTM), at FNUSA-ICRC. Dr. Vinciguerra authored more than 110 papers and he joined ICRC after having held positions at the University of Geneva, the European Molecular Biology Laboratory (EMBL) and the University College London (UCL). The laboratory is broadly interested in the epigenetics of nutrition and cancer. Current projects include:

  1. Study of epigenetic pathways involving macroH2A1, a variant of histone H2A, a repressor of gene transcription in the differentiation of liver cancer stem cells.
  2. Study of macroH2A1 in the differentiation of hematopoietic stem cells.
  3. Study of the interplay between diet, immune system and gut microbiota during aging.

To achieve these goals we use various in vitro and in vivo models and we generated/will generate a large quantity of next generation sequencing data (RNA-Seq, ChIP-Seq, metagenomics, microarrays), which are fundamental to understand mechanisms and identify druggable targets. The successful candidate will analyze and functionally interpret NGS data.

Key References:

• Sheedfar F, Vermeer M, Pazienza V, Villarroya J, Rappa F, Cappello F, Mazzoccoli G, Villarroya F, van der Molen H, Hofker MH, Koonen DP, Vinciguerra M. (2015) Genetic ablation of macrohistone H2A1 leads to increased leanness, glucose tolerance and energy expenditure in mice fed a high- fat diet. Int J Obes (Lond). 39(2):331-8.

• Rappa F, Greco A, Podrini C, Cappello F, Foti M, Bourgoin L, Peyrou M, Marino A, Scibetta N, Williams R, Mazzoccoli G, Federici M, Pazienza V, Vinciguerra M. (2013) Immunopositivity for histone macroH2A1 isoforms marks steatosis-associated hepatocellular carcinoma. PLoS One. 8(1):e54458.

• Borghesan, M, Fusilli, C, Rappa, F, Panebianco, C, Rizzo, G, Oben, JA, Mazzoccoli, G, Faulkes, C, Pata, I, Agodi, A, Rezaee, F, Minogue, S, Warren, A, Peterson, A, Sedivy, JM, Douet, J, Buschbeck, M, Cappello, F, Mazza, T, Pazienza, V, Vinciguerra, M (2016) DNA hypomethylation and histone variant macroH2A1 synergistically attenuate chemotherapy-induced senescence to promote hepatocellular carcinoma progression. Cancer Res 76(3):594-606.

• Pazienza V, Panebianco C, Rappa F, Memoli D, Borghesan M, Cannito S, Oji A, Mazza G, Tamburrino D, Fusai G, Barone R, Bolasco G, Villarroya F, Villarroya J, Hatsuzawa K, Cappello F, Tarallo R, Nakanishi T, Vinciguerra M (2016) Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis. Epigenetics Chromatin. 25;9:45.

About FNUSA-ICRC

The International Clinical Research Center of St. Anne's University Hospital in Brno (FNUSA-ICRC), Czech Republic is a new-generation research center focusing on the pathogenesis of diseases including finding new methods, technologies and medicaments for effective prevention, early diagnostics and individualized treatment.

We are a top Central European research center with more than 200 researchers, new technologies and experience in using EU grant support. We focus on research and development mainly in the fields of cardiovascular and neurological diseases. Our partners are world-class research centers and universities.

You already have:

  • PhD degree in relevant discipline, such as Bioinformatics, Biostatistics, Structural Biology/Epigenetics
  • Basic knowledge of physiology and pathology, and curiosity to learn.
  • Expertise in handling big data (RNA-Seq, ChIP-Seq, metagenomics etc)
  • Knowledge of at least one among R, python, perl
  • Expertise in data retrieval from public sources
  • Experience in using open-source bioinformatic software (https://en.wikipedia.org/wiki/List_of_open-source_bioinformatics_software), as well as commercial ones (PARTEK, Ingenuity Pathway Analysis and others).
  • A proven record of publication in reputable scientific journals

You are:

  • A team oriented person with a good knowledge of English, detail oriented and meticulous.
  • An excellent communicator, with a strong desire for career development.
  • Available to occasionally travel in other European for training and collaborative experimental efforts within the project

We offer:

  • Participation in international medical research
  • Professional support for your grant applications
  • Short-term and long-term internships abroad
  • Truly international work environment
  • Close cooperation with wide range of stake-holders from top researchers and clinicians to opinion leaders in pharmaceutical/biotechnological industry
  • Position where you will contribute to addressing the major health issues of today
  • Opportunity to make an impact in a young and dynamic organization
  • Mentorship, Competitive salary, 5 weeks of vacation

How to apply:

The candidates are invited to send their CV and motivation letter to kristina.chuda@fnusa.cz.

Disclaimer:

We are pleased to consider all qualified applicants for employment without regard to age, gender, marital status, sexual orientation, race, color, religion, ethnicity, disability, national origin, nationality, political opinion or any other aspect unrelated to work.

By responding to this invitation and/or sending your CV and any other personal materials to St. Anne's University Hospital, you consent to the collection, processing and storage of your personal data in accordance with Act No. 101/2000 Coll., the protection of personal data. You are providing St. Anne's University Hospital with these data solely for the purpose of mediating employment, the period however being no longer than one year from submitting. Should you not reclaim the materials submitted by you, they will be discarded after the period has expired.