Center of Biomolecular and Cellular Engineering (CBCE)

Principal Investigators:

Mgr. Kamil Paruch, Ph.D. - CBCE Group Leader; Medical Chemistry (MCHEM)

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doc. Mgr. Petr Beneš, Ph.D.  - Tumor Microenvironment and Metastasis (TMM)

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prof. Mgr. Jiří Damborský, Dr.  -  Protein Engineering (PEG)

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prof. Ing. Petr Dvořák, CSc.  -  Stem Cells and Cell Signaling (SCCS)

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doc. MVDr. Aleš Hampl, CSc.  -  Cell and Tissue Regeneration (CTR)

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doc. Mgr. Lumír Krejčí, Ph.D.  -  Genome Integrity (GENI)

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Mgr. Lukáš Kubala, Ph.D.  -  Inflammation (INF)

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Mgr. Karel Souček, Ph.D.  -  Cancer Plasticity (CAP)

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Mgr. Stjepan Uldrijan, CSc.  -  Cancer Metabolism (CAME)

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Contact to Assistant

Bc. Ivana Hanáková, e-mail: ivana.hanakova@fnusa.cz, phone: +420 549 491 392


Research team


Profile

Biologically active small molecules and engineered proteins currently represent the most promising tools for influencing biological processes such as genomic cell stability, cell growth, cell differentiation, cell reprogramming to pluripotency, cell death, and cell survival. In this program, we will implement strategies based on the definition of biologically important targets, rational design and synthesis of chemical compounds, and well-established testing systems to manipulate these processes in living cells with a high degree of control.

Main research areas

  • Research on pluripotent stem cells - differentiating stem cells, modeling genetic diseases, genomic instability, technology and the use of induced pluripotent cells.
  • Medicinal chemistry - development of patentable, biologically active organic compounds that can serve as starting points for preclinical research programs with a focus on the cancer treatment sector.
  • Cell signaling - research on the mechanism of cancer development, expertise in flow cytometry, high-speed sorting.
  • Cellular differentiation - research on the molecular principles of proliferation, differentiation, migration and programmed death of animal and human cells and their changes in tumor cells.
  • Protein engineering - focus on enzymatic catalysis of the environment as well as chemical, biomedical, and military/defense applications.
  • Recombination and DNA repair - research on DNA repair processes with relevance to cell cancer, repairs of damaged DNA, and recombination with a focus on identifying inhibitors regulating individual repair pathways and their potential use in the treatment.
  • Redox biology and medicine - research on damage to organisms and ways to prevent it, primarily by influencing physiological processes (free radical signaling).
  • Tissue engineering - regenerative medicine and in vitro testing of bioactive compounds on stem cells.
  • Tumor biology - cancer research with the possibility of targeted treatment of cancer, especially malignant melanoma.

Technology background

  • Automatic cell imaging system with medium to rather high throughput - a technological module, which is part of the laboratory capable of producing human pluripotent stem cells of the quality that is adequate for the cells to be safely expanded, characterized, produced, stored and tested robustly and reproducibly.
  • Cellular imaging laboratory - focuses on the use of live event imaging techniques at the microscopic level. Among the key devices, there are, e.g., the laser scanning microscope, which is capable of monitoring events in the four-dimensional space-time in cell cultures and tissues, whole-body luminometry enabling tracking of individual stem cells labeled by vector-luminescence luciferase system in an animal model at the whole body level, etc.
  • Laboratory of cellular separations and cultures - used for separating stem and other cells in high purity and their cultivation for research purposes in cellular therapy. The key instrument of the laboratory is the cell sorter, which allows to analyze the cells by 10 parameters simultaneously while sorting them using the same without damaging their viability.
  •  Laboratory for cytomics, proteomics and genomics - analyzes the results of cultivating stem cells and their differentiation using specialized methods at the level of cells, proteins, and genome. The lab has a flowcytometer available - an automated system for analyzing particles with the use of fluorescently labeled antibodies and dyes, which allows simultaneous analysis of up to 10 parameters in a single cell using laser beams.

Projects

  • FNUSA-ICRC Science for Health: Support of Clinical Research Popularization (ECOP)
  • HISTOPARK - Centre for analysis and modeling of tissues and organs (ECOP)
  • Human Bridge for Strengthening Integration of ICRC into European Research Area (ICRC-ERA Human Bridge, FP7)
  • ICRC Human Bridge - Support of Study Stays of Czech Researchers Abroad III: Young Talent Incubator (ECOP)
  • ICRC PartnerNet - Research Partnership Network (ECOP)
  • MEDINFO - Information Sources for Medicine and Related Fields (R&DI OP)
  • OrganoNET - partnership for education and reseach in the field of visualisation of tissues and organs (ECOP)
  • PRECEDENCE (R&DI OP)

Publications